ANTI-Phospho-Bad-S75 Antibody

Analysis Note

ControlWestern Blot : RAW264.7/LPS lysate.

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-Phospho-Bad (Ser75) Antibody detects level of Phospho-Bad (Ser75) & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Immunogen

This antibody is generated in rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS75 of human Bad.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulins in PBS with 0.09% NaN3.

Format: Purified

Protein G followed by IAP

Quality

Western Blot Analysis: 1:250 dilution of this antibody detected phospho-Bad (Ser75) in lysate from LPS-stimulated RAW264.7 cells.

Specificity

Other species have not been tested.

This antibody reacts with the 18 kDa phospho-Bad (Ser75) protein.

Storage and Stability

Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C

Target description

18 kDa